抄録
Fetal bovine serum (FBS), commonly used in the culture of many insect cell lines, is a source of both nutrients and bioactive compounds that promote cell growth; however, because the growth-promoting compounds contained in FBS are not considered to be identical to those produced by insects, the response of insect cells to FBS is likely to differ from responses to endogenous growth-promoting compounds. The present study examined whether primary cultures of Bombyx embryonic tissue fragments respond differently to silkworm egg extract and FBS. It was observed that, in primary cultures, Bombyx egg extract promoted the migration and growth of cells from embryonic tissue fragments more potently than FBS. These results indicate that the extract of silkworm eggs in an early developmental stage contains molecules that strongly promote cell migration and growth. The use of egg extracts could also shorten the time required to establish a novel cell line.
