Applied Entomology and Zoology
Online ISSN : 1347-605X
Print ISSN : 0003-6862
ISSN-L : 0003-6862
Effect of Larval Extract and Proteinase K on the In vitro Infectivity of Heliothis zea Nonoccluded and Alkali-Liberated Virions
Arthur H. MCINTOSHCarlo M. IGNOFFO
著者情報
ジャーナル フリー

1988 年 23 巻 2 号 p. 175-180

詳細
抄録
Proteinase K and extracts of virus-diseased larvae (LX) significantly reduced the in vitro viral titers of the nonoccluded virus (NOV) of the single-enveloped nuclear polyhedrosis virus of Heliothis zea (HzSNPV). Similar treatment of alkali-liberated occuluded virions (LOVAL) increased the viral titer by as much as 2.5 logs. Alkali dissolution of heat treated polyhedral inclusion bodies (PIB) of HzSNPV with sodium carbonate consistently resulted in high titers ranging from 105.3 to 106.9 TCID50/ml.
著者関連情報
© the Japanese Society of Applied Entomology and Zoology
前の記事 次の記事
feedback
Top