Collection and Hatching Methods of the Aphid, Aphis spiraecola PATCH, Eggs in the Laboratory

抄録

The best method for collection and hatching of eggs of Aphis spiraecola was studied in the laboratory. Almost 80% eggs were deposited during the first seven days. Most eggs were obtained when the cotton cloth was used as the oviposition surface and aphids were maintained at 20°C. However, eggs on the cotton cloth did not pigment as fast as those on the filter paper, and the final percentage of pigmentation was lower on the cotton cloth than on the filter paper. In addition, eggs on the cotton cloth easily detached from surface when eggs were dipped in water. It is concluded that the filter paper is better surface for egg collection. The highest percentage of pigmented eggs (90%) gained when eggs on the filter paper were maintained at 14°C. Females with an artificial diet did not produce more eggs than those without it. Sterilization by 1% formaldehyde solution treated immediately after oviposition had the ill effect for the egg pigmentation. Prolonged pre-cooling incubation period at 14°C did not affect post-diapause development of eggs.