ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
TECHNICAL ADVANCEMENT
Coherent Anti-Stokes Raman Scattering Microscopy
Mamoru Hashimoto
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2002 Volume 35 Issue 2 Pages 83-86

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Abstract
In this paper, new multiphoton microscopy using CARS (coherent anti-Stokes Raman scattering) spectroscopy is described. CARS microscopy has the features of non-staining molecular mapping by molecular vibration imaging and three-dimensional resolution by multiphoton process. A picosecond tunable laser and suitable optical filters provide the CARS imaging in the fingerprint region, and multi-focus excitation using a rotatory-microlens array enables the multi-spectral imaging.
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© 2002 By the Japan Society of Histochemistry and Cytochemistry
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