Antigen Retrieval on Ultrathin Cryosections

Abstract

We have studied two different monoclonal antibodies that behave poorly for the immunocytochemical localization of caveolin-1 and caveolin-2 in paraformaldehyde fixed tissue. Treatment of tissue sections with sodium dodecyl sulfate (SDS) led to enhanced immunocytochemical detection of antigens with each of these antibodies. In this study, we have focused on the use of SDS for antigen retrieval using ultrathin cryosections. Herein, we show that SDS treatment can be applied to ultrathin cryosections without deleterious effects and can be used for high-resolution immunofluorescence detection of caveolin-1 and -2.