Abstract
Immunolabeling of cultured cell lines of epithelial origin is often performed to study cell polarity. To explore the localization of cellular molecules in detail, we devised an efficient and convenient method to prepare semithin and ultrathin cryosections of cells cultured on permeable support. Multiple specimens were piled up to obtain many vertical images of cells in a single section. Freezing them in cooled n-hexane resulted in clear and homogeneous frozen specimens, from which semithin and ultrathin cryosections of epitherial cells on permeable support were easily obtained. With this method, it is possible to prepare many cryosections of good quality for histochemical examination of vertical images of cells.
