ARYLSULFATASE HISTOCHEMISTRY I. VARIATIONS IN FIXATION AND PH FOR DETECTION OF REACTION PRODUCT WITH P-NITROCATECHOL SULFATE AS SUBSTRATE IN THE PRESENCE OF LEAD IONS

Abstract

A modification of Goldfischer's method for demonstration of activity for arylsulfatase has been employed. At pH 6.5 two forms and localizations of reaction product can be distinguished in the kidney: A supranuclear granular reaction and a diffuse cytoplasmic staining. In the liver only discrete granules are present. The nature of the reaction product depends considerably on fixation and pH. At pH 8.0 non-specific precipitation takes place. At pH lower than 5.5 no reaction product is formed.