Abstract
The localization of amylase and deoxyribonuclease (DNase) in rat parotid gland was investigated on both light and electron microscopic levels by use of the immunohistochemical method.
Acrylamide gel embedding method was developed to prepare both ultrathin sections for electron microscopic observation and the sections for light microscopy from an identical tissue block.
Both amylase and DNase localized in the secretory granules of all acinar cells at a constant concentration, but they were not recognized in duct cells. Under electron microscopic observation, these enzymes were distributed in the secretory granules and condensing vacuoles of acinar cells. Both enzymes were demonstrated simultaneously in the identical secretory granules by using immunoferritin and immunoperoxidase methods.
