ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
IMMUNOHISTOCHEMICAL STUDY OF AMYLASE AND DEOXYRIBONUCLEASE IN RAT PAROTID GLAND
SHUJI YAMASHITA
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1981 Volume 14 Issue 3 Pages 236-260

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Abstract
The localization of amylase and deoxyribonuclease (DNase) in rat parotid gland was investigated on both light and electron microscopic levels by use of the immunohistochemical method.
Acrylamide gel embedding method was developed to prepare both ultrathin sections for electron microscopic observation and the sections for light microscopy from an identical tissue block.
Both amylase and DNase localized in the secretory granules of all acinar cells at a constant concentration, but they were not recognized in duct cells. Under electron microscopic observation, these enzymes were distributed in the secretory granules and condensing vacuoles of acinar cells. Both enzymes were demonstrated simultaneously in the identical secretory granules by using immunoferritin and immunoperoxidase methods.
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© the Japan Society of Histochemistry and Cytochemistry
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