PRE-EMBEDDING IMMUNOELECTRON MICROSCOPY BY PEROXIDASE-LABELED ANTIBODY METHOD APPLICATIONS TO ENDOCRINOLOGY

R. YOSHIYUKI OSAMURA; NORIYUKI KOMATSU; SHINICHI IZUMI; SHINICHI YOSHIMURA; MASANORI MURAKOSHI; KEIICHI WATANABE

doi:10.1267/ahc.14.383

PRE-EMBEDDING IMMUNOELECTRON MICROSCOPY BY PEROXIDASE-LABELED ANTIBODY METHOD

APPLICATIONS TO ENDOCRINOLOGY

R. YOSHIYUKI OSAMURA, NORIYUKI KOMATSU, SHINICHI IZUMI, SHINICHI YOSHIMURA, MASANORI MURAKOSHI, KEIICHI WATANABE

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JOURNAL FREE ACCESS

1981 Volume 14 Issue 4 Pages 383-390

DOI https://doi.org/10.1267/ahc.14.383

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Abstract

Pre-embedding immunoelectron microscopy has demonstrated the ultra-structural localization of antigens in various intracellular organelles, including cisternae of rough endoplasmic reticula, pen-nuclear spaces, Golgi saccules, cytosol, secretory granules and mitochondria.
In this report, major staining procedures of this method are briefly described and its applications to endocrinology are demonstrated.

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