Abstract
For the demonstration of acetylcholine receptor (Ach R) at neuromuscular junction using α-bungarotoxin-horseradish peroxidase (α-BT-HRP) conjugate, a modified conjugation method was described to keep the better binding activity to Ach R. For the tissue processing, three methods were compared regarding the binding specificity and preservation of ultrastructure. The best result was obtained when EPON sections were made from glutaraldehyde-fixed tissue after incubation with α-BT-HRP conjugate. The preliminary examination of cryostat sections and subsequent embedding for electron microscopic examination was useful but unsatisfactory for the preservation of membrane structure. Electron microscopic examination revealed that α-BT was localized both on the presyraptic and on the postsynaptic membrane.
