Abstract
Various techniques of fluorescence histochemistry have some methodological difficulties mainly due to the instability of amine fluorophores. The present study aims to develop a stable and relatively simple histofluorescence method which can be applied to the routine examination for diagnostic histopathologists.
Dissected tissue pieces were fixed by immersing in a FAGLUPAGAS* solution overnight. Cryostat sections of fixed materials were washed in a FAGLUGAS solution, mounted on glass slides, dried by warm air and finally cover-slipped in Entellan.
Amine fluorescences in thusly fixed tissues were stable for several months when the specimens, either tissue blocks or cryostat sections, were kept in the FAGLUPAGAS.
