Abstract
Taking γ-glutamyl transpeptidase (γ-GT)-monoclonal anti γ-GT system as an example, the effect of microwave fixation for the immunohistochemical study was examined in rat kidney, pancreas, duodenum and liver, using a domestic microwave oven. The results were compared with that of the previous study using conventional fixatives. Best results were obtained in tissues irradiated in 4%-8% paraformaldehyde containing 10% glucose or 5%-10% polyethylene glycol 2000 at 45°-50°C. But, the immunoreaction was not demonstratable along the brush border of the duodenum. Though the microwave fixation is a useful tool for immunohistochemistry, it is necessary to adjust the condition of irradiation and the composition of fixatives to keep the physiological state of each organ or tissue well preserved and for the antigen to stay as it is originally located without loosing its antigenicity.
