Abstract
A simple and new technique was developed to ensure intimate contact of film with whole-body sections mounted on glass slides. Mice were injected with 125I-labeled insulin intravenousely. Whole-body frozen sections were prepared using adhesive tape along with a piece of dry paper and transferred to cold glass slides coated with egg albumin and glycerin. After freeze drying, a slide-mounted section was placed in a plastic bag, and brought into contact with the film. The bag was subsequently evacuated and sealed by a vacuum sealer. By this method, film maximum contact could be maintained with the section during exposure. Following development of the film, the section was stained and used for histological identification of tissues in the autoradiograph.
