A SIMPLE G-BANDING TECHNIQUE ADAPTABLE FOR FLUORESCENT IN SITU HYBRIDIZATION (FISH) AND PHYSICAL ORDERING OF HUMAN RENIN (REN) AND CATHEPSIN E (CTSE) GENES BY MULTI-COLOR FISH

Abstract

We developed a simple technique for delineation of refined replication G-band in combination with fluorescent in situ hybridization (FISH). By using this system we localized three genes of pepsinogen C (PGC), renin (REN), and cathepsin E (CTSE) on 6p21.1, 1q32, and 1q32, respectively. Further, we determined the physical order of REN and CTSE on chromosome band 1q32 as cen-(1q32)-REN-CTSE-tel by multi-color FISH.