Abstract
A simple and useful double staining method has been developed for light microscopic immunohistochemistry. The technique consisted of a sequence of protein A-gold staining followed by a single silver enhancement procedure, and in this technique reaction products were colored black, and brown or reddish purple, respectively. The results obtained in the rat pancreatic islets and adenohypophysis in combination with a variety of histochemical controls have substantiated the specificity, reliability and reproducibility of the double staining method. The present method is simple and economical, since visualization of the reaction products of two different shades can be obtained by a single procedure.
