Abstract
Dewaxed and hydrated sections were treated with a 0.5% acriflavine hydrochloric acid solution of pH 1.5 for 30 min and then washed with distilled water followed by a rinse in descending concentration of ethanol and again in water. The sections were next treated with a 0.1% alcian blue acetic acid solution of pH 2.5 for 2-20 min, rinsed in water, dehydrated with ethanol and then cleared and mounted. Cartilage matrix appears bluish yellow and lacunar borders and chondrocyte cytoplasm yellowish blue. Mast cell cytoplasmic constituents appear bluish orange in contrast to the pale yellow of the nucleus.
