Abstract
The distribution of lactate dehydrogenase (LDH) subunits was investigated in normal bovine tissues such as heart, kidney, liver, and skeletal muscle using fluorescent antibody technique. Fluorescence specific for LDH-H was located in the heart muscle fibers, in the cytoplasm and membrane of hepatic cells, and in the cytoplasm of epithelia of distal tubuli. Specific fluorescence of LDH-M was observed in the skeletal muscle fibers and in the cytoplasm of hepatic cells. Immunofluorescence was in accord with the results of histochemical localization by nitro blue-tetrazolium stain. Cold acetone was the best fixative among those examined. But it failed to locate LDH-M in renal tissue, and this observation was discussed.
