Abstract
In this study, I used an Olympus single beam microfluorimeter which was modified from the multi-purpose single beam microspectrophotometer according to my specifications.
This instrument was used to the sample the accuracy of localization of mouse lens crystallins by direct and indirect immunofluorescence methods. Some of the problems involved in microfluorimetry include: the necessity of the constant intensity of the exciting light, the choice of the filter system and the spot exciting method.
From a practical point of view it was concluded that the indirect method of immunofluorescence proivdes a more accurate localization of each lens crystallin than the direct method.
