Abstract
Four kinds of hydrolytic enzymes, alkaline and acid phosphatases, leucine aminopeptidase and nonspecific esterase, were applied to cytological specimen for cytochemical diagnosis of carcinoma. Alkaline phosphatase is positive in normal columnar and reserve cells of uterine cervix, negative in normal squamous cells and irregularly positive, though decreased in reaction, in dysplasia and squamous carcinoma cells. This findings contribute to the idea of cytogenesis of cervical carcinoma of uterus as well as to practical cytological diagnosis. Four kinds of enzymes were also stained on the smear of metastatic effusion of gastric, pulmonary, ovarian, endometrial and mammary carcinoma. Cytochemical classification was designed to express peak and range of enzymatic distribution. Cytochemical patterns, thus represented, are instrumental to identify unknown primary site of tumor from ascitic and pleural fluid.
