FEULGEN NUCLEAL REACTION III. A CONTRIBUTION TO THE STUDY OF THE ADSORPTION ISOTHERM AND STOICHIOMETRY OF FEULGEN'S NUCLEAL REACTION IN SITU

Abstract

In the hope of gaining some insight into the staining mechanism of the Feulgen nucleal reaction in situ, the staining reaction of three kinds of hydrolyzed nucleoprotein-containing films with Schiff's reagent was studied from both the physicochemical and the chemical points of view. The establishment of an approximate equilibrium state in staining was assumed, and the mode of the adsorption isotherm in this state was examined. The Feulgen nucleal reaction in the heterogeneous phase conforms to Langmuir's adsorption isotherm and thus exhibits a saturation value in staining. Based on this fact, the stoichiometry of the reaction was determined. The saturation value of the stoichiometry was proved to be four.