In this study, the details of the existing physiological models of plant transpiration, Jarvis and Kosugi models, were reviewed and field experiments were also conducted to investigate the influence of environmental factors on the transpiration of trees, particularly roadside trees. Data were collected by measuring the environmental factors and the factors influencing stomatal transpiration. The following results were obtained: (1) The transpiration of trees during the daytime was most significantly influenced by solar radiation or photosynthetically active radiation, followed by the leaf or air temperature. (2) The influence of the relative humidity of air on the total amount of transpiration of trees during the daytime was not significant.(3) On the prediction precision of the two models on plant transpiration, Jarvis and Kosugi models were almost the same.