It is necessary to prepare a plenty of subjects in order to ensure the statistical confidence in the sensory evaluation of odor. Therefore, it is convenient if the minimum number of subjects can be prepared according to the necessary accuracy of measurement. In this paper, the sensory evaluation experiment with 1-butanol was conducted and evaluations by six subjects were compared with those by sixty subjects under the same sixty votes in total. Moreover, confidence intervals of intensity and percentage of person dissatisfied were estimated, and relationships between confidence intervals and the number of subjects were indicated.