Precast prestressed concrete (PCaPC) beams with a pretensioning system are often used for constructing long-span reinforced concrete buildings. It is important to clarify the bond characteristics of the prestressing strands, because the concrete is prestressed through the bond between the strands and the surrounding concrete. A bond test and a loading test were carried out to clarify the bond characteristics and the structural performance of PCaPC beams using large diameter strands. As a result, the anchorage length of strands for prestressing and after large deformation, and the load-displacement relationship of the PCaPC beam under seismic loading were clarified. Based on these results, it is concluded that large diameter strands can be used as prestressing steel in PCaPC beams.