The authors previously presented an elasto-plastic analytical method of concrete-filled steel tubular (CFT) columns in consideration of fluctuation of axial force. In this paper, we conducted a seismic response analysis and an incremental loading analysis of a 40-story plane frame in which CFT columns are used. We compared the results between the proposed analysis model and a conventional one. In the results, the proposed model could simulate the elasto-plastic behavior of CFT columns more precisely. We show effectiveness of this analytical method for structural design of high-rise buildings.