The biotope which considered selection of the zoning of a planting area, the large water area with ecotone, and introduced animals was built in the private corporation site in the center of Tokyo so that the good living thing habitat could be created. The following things became clear by investigation for four years. Many insects and birds which appeared used this biotope as not only a rest place or a feeding area but also a nest building place. The introduced animals contributed to invitation of animals appeared there. Many introduced animals suited this environment, and though the high predation pressure of the animals appeared was received, several introduced animals increased the numbers of individuals. Also it became clear that the environment which surrounded the biotope was not necessarily gentle to the animals appeared, for example, no installation of animal pathways for them.