The authors designed and constructed two types of tensegrity structure, tower tensegrity and truncated pentagonal prism tensegrity, as a structural skeleton to support a membrane roof. In the Part 1, the progress of design and manual pretensioning scheme of the tower tensegrity, and results of practical performance tests of manual pretensioning in a laboratory were reported. In the Part 2, the state and result of pretensioning of the tower tensegrity during the actual construction is reported and compared to the predicted behavior in the design stage.