In this study, evaluation methods of subgrade reaction for horizontal resistance analysis of piles using the beam-spring model were studied by simulation analysis of horizontal loading test results of pile. As a result, the analysis for pile head horizontal load obtained results on the safe side when the method of the guideline was used for an evaluation of subgrade reaction. On the other hand, the analysis using an evaluation method of coefficient of subgrade reaction proposed by the authors and an evaluation method of plastic subgrade reaction proposed by Kishida-Nakai produced results that generally corresponded to the measured values.