Experiments were conducted to evaluate the withdrawal performance of screwed joints for diagonal beam fastening structures at the joints between diagonal beams and beams, which serve as tensile members in truss structures used in wooden buildings with large spaces. In the experiment, the angle of the diagonal beam, the angle of the screw, the length of the screw, and the direction of the screw were used as parameters. The experimental results were in general agreement with the calculation results using the proposed formula for inclined screw withdrawal strength.