抄録
The cell infiltration in breast carcinoma (stromal reaction) and the reactive changes in the axillary lymph nodes were investigated and assessed together with the production of factor inhibiting Ehrlich ascites-cell migration and suppressor factor. We used methods of morphology, immunohistochemistry and migration inhibition assay were applied. The study comprised 144 women with breast carcinoma. Their age at operation varied from 32 to 70 years with the mean (standard deviation) of 50.5 (9.9) years. Among 742 axillary lymph nodes from 144 patients, 125 axillary lymph nodes from 32 patients did not react and we examined the remaining 617 axillary lymph nodes from 112 patients. Reactive changes were found in 282 (45.7%) of these lymph nodes, most of which were in T-cell region (paracortical type) and T-cell and B-cell region (mixed type). The active paracortex in the lymph nodes was related with production both of a factor inhibiting Ehrlich-cell migration and a suppressor factor. We have shown that inhibitory factor was produced from erythrocyte rosette-forming cells and the subset TR (rerosetting T-cells after incubation of theophylline) was stimulated in vitro with breast-cancer extract. The erythrocyte rosette-forming cells were separated from peripheral blood mononuclear cells and lymph node lymphocytes. The cells found in the subset TS (non-rerosetting T-cells) produced suppressing factor. The latter could inhibit the proliferation and differentiation of CD8+ T-cytotoxic lymphocytes, and possibly damage their function.
