Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Incorporation of Horseradish Peroxidase in a Kieselguhr Membrane and the Application to a Mediator-free Hydrogen Peroxide Sensor
Chunhai FANHaiyan WANGDexu ZHUGerhard WAGNERGenxi LI
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2001 年 17 巻 2 号 p. 273-276

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Horseradish peroxidase was incorporated in a kieselguhr membrane. The electron-transfer process of the enzyme was examined by cyclic voltammetry. It was observed that the electron-transfer reactivity of horseradish peroxidase was greatly enhanced, and that direct electrochemistry was accordingly feasible. Using the merits of the direct electron-transfer reactivity of horseradish peroxidase and its specific enzymatic catalysis towards hydrogen peroxide, an unmediated hydrogen peroxide biosensor was constructed. The calibration plot of this hydrogen peroxide sensor was linear in the range of 2.0 × 10-6 mol/L - 6.5 × 10-4 mol/L. The relative standard deviation was 4.1% for 6 successive determinations at a concentration of 1.0 × 10-4 mol/L. The detection limit was 1.0 × 10-6 mol/L.

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© 2001 by The Japan Society for Analytical Chemistry
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