Enantiomer Separation of Miconazole by Capillary Electrophoresis with Dual Cyclodextrin Systems

Abstract

A cyclodextrin-modified micellar capillary electrophoretic method (MECC) was developed using mixtures of β-cyclodextrins (β-CD) and mono-3-O-phenylcarbamoyl-β-CD as chiral additives for the chiral separation of miconazole with the dual CDs systems. The enantiomers were resolved using a running buffer of 50 mmol/L borate pH 9.5 containing 15 mmol/L β-CD and 15 mmol/L mono-3-O-phenylcarbamoyl-β-CD containing 50 mmol/L sodium dodecyl sulfate and 1 mol/L urea. A study of the respective influence of the β-CD and the mono-3-O-phenylcarbamoyl-β-CD concentration was performed to determine the optimal conditions with respect to the resolution. Good repeatability of the method was obtained.