Flow-Injection Determination of <font size=-1>L</font>-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection

Nobutoshi KIBA; Ayumi KOGA; Masaki TACHIBANA; Kazue TANI; Hitoshi KOIZUMI; Tetsuo KOYAMA; Akira YAMAMURA; Kunio MATSUMOTO; Tohru OKUDA; Koki YOKOTSUKA

doi:10.2116/analsci.22.95

Original Papers

Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection

Nobutoshi KIBA, Ayumi KOGA, Masaki TACHIBANA, Kazue TANI, Hitoshi KOIZUMI, Tetsuo KOYAMA, Akira YAMAMURA, Kunio MATSUMOTO, Tohru OKUDA, Koki YOKOTSUKA

Author information

Nobutoshi KIBA
Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi
Ayumi KOGA
Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi
Masaki TACHIBANA
Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi
Kazue TANI
Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi
Hitoshi KOIZUMI
Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi
Tetsuo KOYAMA
Department of Applied Chemistry, Faculty of Engineering, Kanagawa Institute of Technology
Akira YAMAMURA
Department of Applied Chemistry, Faculty of Engineering, Kanagawa Institute of Technology
Kunio MATSUMOTO
Department of Applied Chemistry, Faculty of Engineering, Kanagawa Institute of Technology
Tohru OKUDA
Laboratory of Biofunctional Science, The Institute of Enology and Viticulture, University of Yamanashi
Koki YOKOTSUKA
Laboratory of Biofunctional Science, The Institute of Enology and Viticulture, University of Yamanashi

JOURNAL FREE ACCESS

2006 Volume 22 Issue 1 Pages 95-98

DOI https://doi.org/10.2116/analsci.22.95

Details

Abstract

A chemiluminometric flow injection analytical system for the quantitation of L-histidine is described. Histidine oxidase (EC 1.4.3.—) from Brevibacillus borstelensis KAIT-B-022 was immobilized on tresylated poly(vinyl alcohol) beads and packed into a stainless-steel column. The hydrogen peroxide produced was detected chemiluminometrically by a flow-through sensor containing immobilized peroxidase (EC 1.11.1.7). The maximum sample throughput was 10 h^-1. The calibration graph was linear from 0.05 to 5 mM; the detection limit (signal to noise ratio = 3) was 0.01 mM. The activity of immobilized histidine oxidase reduced to 65% of the initial value after 350 injections. The system was applied to the determination of L-histidine in fish meat, such as salmon, tunny, bonito, and mackerel.

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