Abstract
A novel, rapid and sensitive analytical method is described for determination of ofloxacin and levofloxacin by enhanced chemiluminescence (CL) with flow-injection sampling. The method is based on the CL reaction of the Ce(IV)-Na2S2O4-ofloxacin/levofloxacin-H2SO4 system. The enhanced CL mechanism was developed and the optimum conditions for CL emission were investigated. The CL intensity was correlated linearly (r = 0.9988) with the concentration of ofloxacin (or levofloxacin) in the range of 1.0 × 10-8 - 1.0 × 10-7 g ml-1 and 1.0 × 10-7 - 6.0 × 10-6 g ml-1. The detection limit (S/N = 3) is 7 × 10-9 g ml-1. The relative standard derivation (RSD, n = 11) is 2.0% for ofloxacin at 4 × 10-7 g ml-1 and for levofloxacin at 6 × 10-7 g ml-1. This method has been successfully applied for the determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids with satisfactory results.
