Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Galactose Oxidase Immobilized on Silica in an Analytical Determination of Galactose-containing Carbohydrates
Lyudmila KONDAKOVAVictor YANISHPOLSKIIValentin TERTYKHTat'yana BUGLOVA
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2007 年 23 巻 1 号 p. 97-101

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Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse and continuous modes of a substrate flowing through a column with an immobilized biocatalyst. The linear measurement ranges for galactose-containing carbohydrates concentrations were determined.
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© 2007 by The Japan Society for Analytical Chemistry
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