Detection of Cytochrome c at Biocompatible Nanostructured Au-lipid Bilayer-modified Electrode

Abstract

The present work describes the dectection of cytochrome c (cyt c) at biocompatible aurum (Au) nanoparticle-structured supported bilayer lipid membrane (sBLM) modified with anionic sites. Au nanoparticles were directly deposited through sBLM modified with lauric acid (LA) to build a hybrid device of nanoscale electrode array via potential cycling in 10 mM HAuCl₄ solution containing 0.1 M KCl. The properties of Au nanoparticle-doped sBLM composite were then characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Results indicated that Au nanoparticles grew in voids of the sBLM with size around 20 - 30 nm. With SWV, after optimization, the results of the experiments indicate that the currents of cyt c were linear functions of its concentrations over the range from 1.0 × 10^-7 to 3.2 × 10^-6 M and the limit of detection (LOD, S/N = 3) was 5 × 10^-8 M. The influences of several common base pairs, amino acids and metal ions on determination of cyt c via this Au nanoparticle-doped sBLM composite were relatively low in experiments, suggesting the excellent biocompatibility of this detection method.