Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Advancements in Instrumentation
Microscopic Measurement of Circular Dichroism Spectra
Aira MATSUGAKIHideaki TAKECHIHideaki MONJUSHIROHitoshi WATARAI
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2008 Volume 24 Issue 2 Pages 297-300

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Abstract
A microscope device to measure the circular dichroism (CD) spectra of a specified microscopic region of chiral samples was constructed by combining of a couple of objective lenses and a CCD camera, which was installed in a sample chamber of a commercially available CD spectropolarimeter. By using this apparatus, high quality micro-CD spectra in the 60 × 60 µm region of samples could be measured. Micro-CD spectra of thin film of chiral DNA samples on glass and a natural kidney bean leaf were measured, and the potential of the micro-CD apparatus was successfully demonstrated.
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© 2008 by The Japan Society for Analytical Chemistry
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