抄録
Indole-3-acetic acid (IAA) is a phytohormone of the auxin group and is capable of coordinating the overall processes of plant growth and development. IAA is active in the very low concentration range. Therefore, it is important to quantify IAA in the low concentration range in complex system. In this work, a new spectrofluorometric method for the direct determination of IAA in soil is proposed and discussed. It combines the fluorescence excitation-emission matrices (EEMs) with second-order calibration methods based on the alternating trilinear decomposition (ATLD) algorithm and the self-weighed alternating trilinear decomposition (SWATLD) algorithm. These methodologies fully exploit the second-order advantage of the three-way fluorescence data, allowing the analyte concentrations to be quantified even in the presence of unknown fluorescent interferents. IAA recoveries in soil were determined as 100.6 ± 3.0 and 96.9 ± 1.1% with ATLD and SWATLD, respectively. The limits of detection obtained were 17.6 and 4.6 ng mL-1, and the limits of quantification were 52.9 and 13.9 ng mL-1 with ATLD and SWATLD, respectively.
