2009 Volume 25 Issue 2 Pages 195-200
The enhanced effect of chemiluminescence (CL) of lucigenin (Luc2+) at a flat liquid/liquid interface and at O/W emulsions was demonstrated. The CL was emitted when Luc2+ was transferred from an organic phase to an aqueous one containing NaOH and H2O2. The CL intensity and emission spectrum depended strongly on the kind of organic solvent. We attempted to apply the CL reaction of Luc2+ in the O/W emulsion system to the analysis of H2O2. The available analytical conditions were discussed from the aspects of the kind of organic solvent, the reaction time and wavelength detected.