Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Tip-enhanced Raman Spectroscopy of Lipid Bilayers in Water with an Alumina- and Silver-coated Tungsten Tip
Atsushi NAKATATomonori NOMOTOTaro TOYOTAMasanori FUJINAMI
Author information
JOURNALS FREE ACCESS

2013 Volume 29 Issue 9 Pages 865-869

Details
Abstract

Tip-enhanced Raman spectroscopy (TERS) of supported phospholipid bilayers in an aqueous environment is discussed in this paper. Two bilayer membranes were examined: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). We fabricated alumina- and silver-coated tungsten tips that are very robust in water. There was a large time-dependence in the TERS spectra for the DOPC bilayers, whereas no such time-dependence was observed in the DPPC bilayer spectra under the probe tip. The spectral changes of DOPC bilayers are discussed in terms of the fluidity of the liquid crystalline phase. Time-resolved TERS thus has the potential to characterize inhomogeneity and diffusion in fluidic phospholipid bilayer membranes.

Fullsize Image
Information related to the author
© 2013 by The Japan Society for Analytical Chemistry
Next article
feedback
Top