Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Highly Sensitive and Rapid Assay of Substance P and Streptolysin O in Human Serum Using Immuno-liposomes and Gramicidin Channels
Misato SAKAMOTOAtsushi SHOJIMasao SUGAWARA
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2013 Volume 29 Issue 9 Pages 877-883

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Abstract

A highly sensitive and rapid method for the determination of substance P (SP) and streptolysin O (SLO) in human serum is described. The assay is based on enriching the analyte by agglutination/precipitation of immuno-liposomes and enhancing the fluorescence intensity by gramicidin A channels. A mixture of the immuno-liposomes encapsulating a pH-sensitive fluorescence dye BCECF, gramicidin A and a given concentration of SP (or SLO) is preincubated in a solution and captured on anti-SP (or anti-SLO)-modified cover slips, followed by measuring fluorescence images after removing excess liposomes. The method allowed quantifying SP and SLO in the range from sub-pg mL−1 to pg mL−1, with detection limits of 0.32 pg mL−1 and 8 fg mL−1, respectively. The present method could determine SP and SLO in 50 – 125 times diluted human serum without any extraction steps. The assay can be completed within 60 min.

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© 2013 by The Japan Society for Analytical Chemistry
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