Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
A Sensitive Amperometric Acetylcholine Biosensor Based on Carbon Nanosphere and Acetylcholinesterase Modified Electrode for Detection of Pesticide Residues
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2014 Volume 30 Issue 6 Pages 669-673


A sensitive biosensor based on acetylcholinesterase (AChE) and carbon nanosphere (CNS) immobilized on a glassy carbon electrode was developed for the detection of pesticides by the inhibition of AChE activity. The inhibition effect of the pesticides resulted in a decrease of current response of the acetylthiocholine chloride that was used as a substrate to obtain an electrochemical signal. When applied to the pesticides carbofuran and fenitrothion, the designed biosensor exhibited high sensitivity and low detection limits. The inhibition ratio of carbofuran and fenitrothion were linearly proportional to their concentrations ranging from 0.40 to 4.79 and 6.26 to 125.31 μg/L, respectively. The theoretical detection limits were found to be 0.082 μg/L for carbofuran and 2.61 μg/L for fenitrothion. Furthermore, as the biosensor offers good reproducibility and stability, it could be used for trace detection of pesticides in real samples. Compared with other AChE biosensors, the proposed biosensor was convenient and it exhibited extreme sensitivity to pesticides.

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© 2014 by The Japan Society for Analytical Chemistry
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