Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Rapid Communications
Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism
Tsuyoshi ESAKITsutomu MASUJIMA
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2015 Volume 31 Issue 12 Pages 1211-1213

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Abstract

Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell mass spectrometry opens the door to the analysis of site- and state-specific molecular detection to elucidate precise molecular mechanisms at the single-cell and organelle level.

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© 2015 by The Japan Society for Analytical Chemistry
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