Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules
Sakon RAHONGTakao YASUITakeshi YANAGIDAKazuki NAGASHIMAMasaki KANAIGang MENGYong HEFuwei ZHUGENoritada KAJITomoji KAWAIYoshinobu BABA
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2015 Volume 31 Issue 3 Pages 153-157

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Abstract

Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.

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© 2015 by The Japan Society for Analytical Chemistry
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