Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Microfluidic Device for Coulometric Detection of Organophosphate Pesticides
Jin WANGTakaaki SATAKEHiroaki SUZUKI
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2015 Volume 31 Issue 7 Pages 591-595

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Abstract

A microdevice for coulometric detection of organophosphate pesticides (OPs) was fabricated based on the measurement of the inhibition of an enzyme, acetylcholinesterase (AChE), by OPs. Thiocholine (TCh) produced in the enzymatic reaction of AChE with acetylthiocholine (ATCh) as a substrate was oxidized on a microelectrode array formed in a main flow channel. Volumes of plugs of necessary solutions were measured using a structure consisting of a row of rhombuses formed in an auxiliary flow channel. The plugs were merged and solution components were mixed at a T-junction formed with the main and auxiliary flow channels. A linear relationship was confirmed between the generated charge and the logarithm of the OP (malathion) concentration in a concentration range between 10−6 and 10−3 M with a correlation coefficient of 0.951. The lower limit of detection was 412 nM.

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© 2015 by The Japan Society for Analytical Chemistry
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