Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Yeast-based Biochemical Oxygen Demand Sensors Using Gold-modified Boron-doped Diamond Electrodes
Tribidasari A. IVANDINIHARMESAEndang SAEPUDINYasuaki EINAGA
Author information
JOURNALS FREE ACCESS

2015 Volume 31 Issue 7 Pages 643-649

Details
Abstract

A gold nanoparticle modified boron-doped diamond electrode was developed as a transducer for biochemical oxygen demand (BOD) measurements. Rhodotorula mucilaginosa UICC Y-181 was immobilized in a sodium alginate matrix, and used as a biosensing agent. Cyclic voltammetry was applied to study the oxygen reduction reaction at the electrode, while amperometry was employed to detect oxygen, which was not consumed by the microorganisms. The optimum waiting time of 25 min was observed using 1-mm thickness of yeast film. A comparison against the system with free yeast cells shows less sensitivity of the current responses with a linear dynamic range (R2 = 0.99) of from 0.10 mM to 0.90 mM glucose (equivalent to 10 – 90 mg/L BOD) with an estimated limit of detection of 1.90 mg/L BOD. However, a better stability of the current responses could be achieved with an RSD of 3.35%. Moreover, less influence from the presence of copper ions was observed. The results indicate that the yeast-immobilized BOD sensors is more suitable to be applied in a real condition.

  Fullsize Image
Information related to the author
© 2015 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top