Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
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The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species
Shunsuke TOMITASaki YOKOYAMARyoji KURITAOsamu NIWAKeitaro YOSHIMOTO
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2016 Volume 32 Issue 2 Pages 237-240

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Abstract

A cross-reactive sensor array consisting of polyion complexes (PICs) between anionic enzymes and poly(ethylene glycol)-modified (PEGylated) polyamines has been used to identify the source of mammalian sera. Although the catalytic activity of enzymes was inhibited by PIC formation with PEGylated polyamines, the subsequent addition of sera caused enzyme releases from PICs through competitive interactions between PICs and serum proteins, generating unique response patterns of changes in the enzyme activity. Linear discriminant analysis of the obtained patterns enabled the discrimination of five sera from different mammalian sources.

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© 2016 by The Japan Society for Analytical Chemistry
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