Detection of Methylated DNA on a Power-Free Microfluidic Chip with Laminar Flow-Assisted Dendritic Amplification

Kazuki HASEGAWA; Mutsuyoshi MATSUMOTO; Kazuo HOSOKAWA; Mizuo MAEDA

doi:10.2116/analsci.32.603

Rapid Communications

Detection of Methylated DNA on a Power-Free Microfluidic Chip with Laminar Flow-Assisted Dendritic Amplification

Kazuki HASEGAWA, Mutsuyoshi MATSUMOTO, Kazuo HOSOKAWA, Mizuo MAEDA

著者情報

キーワード: DNA methylation, detection, immunoassay, power-free microfluidic chip, LFDA

ジャーナルフリー
電子付録

2016 年 32 巻 6 号 p. 603-606

DOI https://doi.org/10.2116/analsci.32.603

詳細

抄録

We report on a detection method for methylated DNA on a microfluidic chip, which needs no external power for fluid pumping. The methylated DNA was sandwiched by immobilized probe DNA and an anti-methylcytosine antibody. The fluorescence signal was amplified by our original amplification technology. The detection method was first optimized using a 22-mer DNA sequence, then further validated using a 60-mer DNA sequence adapted from the SEPT9 gene. We were able to detect the methylated 60-mer DNA at 0.4 nM within 18 min.

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