Pneumocandin B₀-imprinted Polymer Using Surface-imprinting Technique for Efficient Purification of Crude Product

Abstract

In this work, we prepared surface molecularly imprinted polymer (MIP) for selective recognition of pneumocandin B₀ (PNB₀). Methacrylic acid (MAA) was first grafted onto silica gel particles (SiO₂) in the manner of “grafting from” by using 3-methacryloxypropyl trimethoxysilane as intermedium, and then PNB₀ molecules were imprinted on the surface of the obtained particles in the presence of ethylene glycol diglycidyl ether as the cross-linker. The prepared MIP-PMAA/SiO₂ was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and thermo-gravimetric analysis, which confirmed the successful grafting of MAA onto SiO₂ and the grafting degree was calculated to be 12.50 wt%. The binding properties of the products were investigated and it is found that the binding process of PNB₀ followed the pseudo-second-order kinetic model. The as-prepared material also displays relatively quick adsorption kinetics and decent recognition affinity toward the template over its structurally related compound.