2017 Volume 33 Issue 10 Pages 1105-1110
A study of an electrochemical sensor based on bovine hemoglobin (BHb) imprinted magnetic nanoparticles (MIPs) was investigated. First, BHb MIPs were successfully synthesized with magnetic Fe3O4@Au nanoparticles as carrier by surface modification of mercapto propionic acid for introducing carboxyl groups, combined with dopamine as the functional monomer and BHb as the template protein. Then, the MIPs were modified to the surface of a glassy carbon electrode (GCE) for electrochemical analysis by using cyclic voltammetry in the potassium ferricyanide solution. Results show that there was a good dynamic response between electrochemical signals and the adsorption amount of protein in the range of 0.05 to 0.5 mg mL−1. Results show that the sensor exhibits a significant specific recognition toward the template protein via selective test and can be used for analysis of serum samples. The synthesized MIPs are suitable for the removal and enrichment of template protein in proteomics. At the same time, the proposed electrochemical sensor can be used for recognition of BHb.