Effect of DNA Methylation on the Velocity of DNA Translocation through a Nanochannel

Xiaoyin SUN; Takao YASUI; Takeshi YANAGIDA; Noritada KAJI; Sakon RAHONG; Masaki KANAI; Kazuki NAGASHIMA; Tomoji KAWAI; Yoshinobu BABA

doi:10.2116/analsci.33.727

Notes

Effect of DNA Methylation on the Velocity of DNA Translocation through a Nanochannel

Xiaoyin SUN, Takao YASUI, Takeshi YANAGIDA, Noritada KAJI, Sakon RAHONG, Masaki KANAI, Kazuki NAGASHIMA, Tomoji KAWAI, Yoshinobu BABA

Author information

Xiaoyin SUN
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
ImPACT Research Center for Advanced Nanobiodevices, Nagoya University
Takao YASUI
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
ImPACT Research Center for Advanced Nanobiodevices, Nagoya University
Japan Science and Technology Agency (JST), PRESTO
Takeshi YANAGIDA
Institute of Materials Chemistry and Engineering, Kyushu University
Institute of Scientific and Industrial Research, Osaka University
Noritada KAJI
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
ImPACT Research Center for Advanced Nanobiodevices, Nagoya University
Japan Science and Technology Agency (JST), PRESTO
Sakon RAHONG
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
ImPACT Research Center for Advanced Nanobiodevices, Nagoya University
Masaki KANAI
Institute of Materials Chemistry and Engineering, Kyushu University
Kazuki NAGASHIMA
Institute of Materials Chemistry and Engineering, Kyushu University
Tomoji KAWAI
Institute of Scientific and Industrial Research, Osaka University
Yoshinobu BABA
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
ImPACT Research Center for Advanced Nanobiodevices, Nagoya University
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Keywords: DNA methylation, single DNA molecule, DNA translocation velocity, nanochannel

JOURNAL FREE ACCESS
Supplementary material

2017 Volume 33 Issue 6 Pages 727-730

DOI https://doi.org/10.2116/analsci.33.727

Details

Abstract

Here, we report the effect of DNA methylation on the velocity of DNA translocation through a nanochannel, as determined by measuring differences in translocation velocities between methylated and non-methylated DNA molecules. We found that the velocity of translocation of methylated DNA was faster than that of non-methylated DNA, which we attributed to variation in the coefficients of diffusion and friction with the nanochannel wall, due to the increased molecular weight and stiffness, respectively, of methylated DNA.

Fullsize Image

Corresponding author

Register with J-STAGE for free!